منابع مشابه
The Aminoacyl Ribonucleic Acid Synthetases
Threonyl ribonucleic acid synthetase was purified 400-fold from rat liver. During stepwise chromatography on diethylaminoethyl cellulose, two fractions with this enzymatic activity were observed. The separated fractions give distinct elution patterns on rechromatography on the same ion exchanger. Threonyladenylate-enzyme complex prepared with 14Cthreonine and 3H-adenosine triphosphate shows a s...
متن کاملSpecies Specificity of Amino Acid Acceptor Ribonucleic Acid and Aminoacyl Soluble Ribonucleic Acid Synthetases.
Several investigators have reported that the activation of amino acids for protein synthesis, i.e. the incorporation of amino acids into soluble ribonucleic acids, is dependent upon the source of both the aminoacyl S-RNA’ synthetases and the S-RNA itself. For example, Rendi and Ochoa (1) reported that aminoacyl S-RNA synthetases from Escherichia coli could not incorporate leucine into yeast or ...
متن کاملAffinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. Cognate transfer ribonucleic acid as a ligand.
The use of tRNA affinity columns for the purification of aminoacyl-tRNA synthetases was investigated. A purification method for valyl-tRNA synthetase from Bacillus stearothermophilus is described that uses two affinity columns, one containing the pure cognate tRNA, and the other containing all tRNA species except the cognate tRNA. A method for the rapid preparation of the two columns was develo...
متن کاملThe three-dimensional structure of yeast phenylalanine transfer ribonucleic acid and its interaction with aminoacyl synthetases.
tertiary structure of the molecule. These seem to be in the region of the ‘hinge’ between dihydrouridine and the ribosylthymine-pseudouridine loop. Further, the n.m.r. studies allow one to propose a detailed structural model for the anticodon loop (Ts’o et al., 1975). The problem of the specificity of aminoacylation has been approached by equilibrium and kinetic studies. A striking feature of t...
متن کاملInhibition of aminoacyl-transfer ribonucleic acid synthetases and the regulation of amino acid biosynthetic enzymes in Neurospora crassa.
Growth conditions that result in the accumulation of the tryptophan intermediate indoleglycerol phosphate or of the histidine intermediate imidazoleglycerol phosphate cause mycelia of Neurospora crassa to exhibit an immediate and sustained increase in the differential rate at which the biosynthetic enzymes of the tryptophan, histidine, and arginine pathways are synthesized. These accumulated in...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1966
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)96612-3